Model Questions for Biochemistry-II

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Model Questions for Biochemistry (Microbiology)

Long Answer Question: (5 × 5 = 25)
1) Explain the biological functions of proteins with examples.
2) Explain the essential amino acids.
3) What is the transamination process? Mention its significance.
4) What are the nucleotides? Explain the biological roles of nucleotides.
5) What are the denaturation and renaturation processes of DNA?

Multiple Choice Question: (1 × 5 = 5) (any five)
1. Marfan's syndrome is thought to be a mutation affecting
A. hemoglobin synthesis                       B. insufficient thyroid production
C. metabolism of homogentisic acid     D. collagen and/or elastin synthesis

2. Protein folding is
A. automatic, mediated by the protein itself
B. mediated by other proteins called chaperones
C. mediated by the ribosomes
D. by the protease enzyme

3. The isoelectric point of an amino acid is defined as the pH
A. where the molecule carries no electric charge
B. where the carboxyl group is uncharged
C. where the amino group is uncharged
D. of maximum electrolytic mobility

4. What is the heaviest of the twenty amino acids?
A. phenylalanine             B. tryptophan
C. tyrosine                       D. histidine

5. D-Alanine and L-Alanine are technically known as
A. polymer                       B. enantiomers
C. epimers                        D. anomers

6. Which of the following pairs of amino acids would carry a negative charge on their side chain at pH 8.0?
A. leucine & glycine              B. asparagine & glutamine
C. histidine & lysine              D. aspartate & glutamate

7. Which of the following amino acid do not fall under the category of essential amino acid?
A. leucine             B. histidine            C. glycine           D. methionine

8. What is the end product of leucine metabolism?
A. acetyl-CoA                            B. pyruvic acid
C. oxaloacetic acid                    D. acetyl carnitine

9. The nitrogen atoms of urea produced in the urea cycle are derived from
A. ammonia                                   B. ammonia and aspartic acid
C. nitrite                                         D. nitrate

10. The products of urea cycle are
A. 1 molecule of urea, 1 molecule of ammonia, 3 molecules of GTP and 1 molecule of fumaric acid
B. 1 molecule of fumaric acid, 1 molecule of urea, 1 molecule of AMP, 2 molecules of ADP
C. 1 molecule of aspartic acid, 1 molecule of ammonia, 1 molecule of fumaric acid, 1 molecule of ATP
D. 1 molecule of urea, 1 molecule of ammonia, 1 molecule of ATP and 1 molecule of fumaric acid

11. Which of the following is used as carbon atom source while producing urea in the urea cycle?
A. arginine                                         B. aspartic acid
C. carbon dioxide                               D. glucose

12. Urea cycle converts
A. ammonia into a less toxic form
B. urea into nitrogen
C. amino acids into ketoacids
D. ketoacids into amino acids

13. The inputs to one cycle of the urea cycle are
A. 1 molecule of aspartic acid, 1 molecule of ammonia, 1 molecule of carbon dioxide, 3 molecules of ATP
B. 1 molecule of urea, 1 molecule of ammonia, 3 molecules of ATP and 1 molecule of fumaric acid
C. 1 molecule of fumaric acid, 1 molecule of urea, 3 molecules of AMP
D. 1 molecule of acetic acid, 1 molecule of urea, 3 molecules of GTP